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. 2021 May 6;11:9686. doi: 10.1038/s41598-021-88956-4

Figure 2.

Figure 2

IgE/OVA and LPS co-exposure leads to expanded antigen-specific CD4+ T cell population. (A) Bone marrow-derived mast cells (BMMCs) from C57BL/6 J mice sensitized overnight with 1 μg/mL anti-OVA IgE were stimulated with 10 μg/ml OVA alone or in combination with 0.1, 1, 10 μg/ml LPS. Unsensitized BMMCs were stimulated with 0.1, 1, 10 μg/ml LPS alone. IL-6 cytokine release from supernatants was assayed by ELISA 6 h post stimulation. (B,C) C57BL/6 J mice received intradermal ear injection of 2W1S peptide and either LPS alone or IgE/OVA. The numbers of 2W1S-specific CD4+ T cells in the cervical draining lymph nodes (B) and spleens (C) were quantified 10 days post immunization. Results are representative of 2 independent experiments (A) and cumulative data from 3 independent experiments with n = 9 mice total (B,C) and shown as mean ± SEM. *P < 0.05 **P < 0.01, **P < 0.001, **** P < 0.0001. Statistical analysis was performed using one-way ANOVA with Tukey’s multiple comparisons test.