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. 2021 May 6;11:9686. doi: 10.1038/s41598-021-88956-4

Figure 3.

Figure 3

Mast cells are required for expansion of antigen-specific CD4+ T cells upon IgE mediated activation and LPS exposure. (A) Mast cell depletion and intradermal ear immunization strategy to quantify the number of 2W1S-specific CD4+ T cells in Mcpt5-Cre + (Cre +) mice and littermates (Cre −) upon 2W1S peptide, IgE/OVA, and LPS stimulation. (B) Immunofluorescent images of mast cells visualized by Avidin-FITC in ear tissue sections from Cre + and Cre − mice to show mast cell reduction in Cre + mice one day post last DT injection taken at 10 × objective. The numbers of 2W1S-specific CD4+ T cells within the cervical draining lymph nodes (C) and spleen (D) of mast cell-depleted (Cre +) mice and littermate controls (Cre −) 10 days post intradermal ear immunization. Results are cumulative data from 3 independent experiments n = 6–11 mice total and shown as mean ± SEM. *P < 0.05 **P < 0.01, **P < 0.001, **** P < 0.0001. Statistical analysis was performed using one-way ANOVA with Tukey’s multiple comparisons test (C) or a Kruskal–Wallis test with Dunn’s correction (D). Scale bar, 500 µm.