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. 2021 May 6;11:9682. doi: 10.1038/s41598-021-88809-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of anti-SARS-COV-2 antibodies as supernatants from hybridoma clones. (a) Antigen specificity of antibody binding from IgG-secreting hybridomas chosen from Series 1–3 immunization schedules. Numbers inside circles are total clones selected, numbers within each portion reflect binding selectivity. (b) Distribution of affinities of the 33 selected clones as measured by BLI. (c) Binding to two different Spike recombinant proteins (ecto Spike trimer and S1 polypeptide) as determined by ELISA. (d) Binding of antibodies to γ-irradiated WA-SARS-CoV-2 by ELISA (15 ng/mL virus plated, supernatant undiluted, secondary antibody = goat anti-mouse IgG HRP conjugate).