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. 2021 May 6;11:9661. doi: 10.1038/s41598-021-88834-z

Table 2.

Morphological differences between plants of C. arabica var. Typica regenerated from mature SEs produced in SE-M9 (osmotic medium) and SE-M3 (non-osmotic) medium after eight months.

Evaluated features Non-osmotic treatment (±SD) Osmotic treatment (±SD)
Number of leaves 12.0 ± 1.2b 22.0 ± 4.0a
Length of leaves (cm) 6.1 ± 0.9b 13.3 ± 1.33a
Leaves width (cm) 2.01 ± 1.7b 4.3 ± 0.5a
Leaves area (cm2) 11.24 ± 0.62b 36.31 ± 3.2a
Number of ribbing (cm) 10.3 ± 1.7b 18.6 ± 1.7a
Length ribbing (cm) 1.16 ± 0.32a 1.88 ± 0.73a
Height (cm) 7.65 ± 1.4b 16.7 ± 1.32a
Internodes (cm) 8.66 ± 1.15a 6.33 ± 1.5b
Length of internodes (cm) 0.92 ± 0.11b 2.0 ± 0.31a
Root length (cm) 23.2 ± 1.24b 47.3 ± 0.9a
Root area (cm2) 11.56 ± 0.08b 105.82 ± 1.06a
Number lateral root 4.7 ± 0.4b 18.3 ± 0.13a
Length lateral root (cm) 12.1 ± 4.7a 16.27 ± 9.13a

Differences in variables among treatments were tested using factorial analysis of variance (ANOVA). Values are means ± standard deviation. In each row, means followed by different letters are significantly different (P < 0.05). SE-M3 non-osmotic medium (control) and SE-M9 osmotic medium. Software ImageJ.