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. 2021 May 6;11:9737. doi: 10.1038/s41598-021-89123-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Inefficiency of NMD substantially contributes to accumulation of Mga SV transcripts in meiotic spermatocytes and spermatids. (A) High expression of Mga SV in spermatocytes and round spermatids was not further elevated by suppressing NMD activity. Non-germ (ESC and MEF) and germ (GSC, SC, and RS) cells were treated with or without CHX for 3 h. Semi-quantitative PCR was performed with cDNAs corresponding to their total RNAs. Full-length gels are presented in Supplementary Fig. S7. (B) Quantification of canonical and splice variant Mga mRNAs in non-germ and germ cells treated with or without CHX. cDNAs used in A were subjected to qPCR to accurately compare their amounts. Data are shown as PSI. Data represent the mean ± standard deviation of three independent experiments. The Student’s t-test was conducted to examine statistical significance. *P < 0.05; ***P < 0.001.