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. 2021 May 6;4:534. doi: 10.1038/s42003-021-02042-z

Fig. 2. MLT addition inhibited early folliculogenesis in in vitro ovary culture system.

Fig. 2

a Identification standards of the activated and atretic follicles. b–d The effect of MLT addition on follicle activation and atresia. n = 5 (Con), 4 (10−8 M MLT), and 3 (10−7 M MLT) biologically independent ovaries, respectively. b Experimental design. c Representative photographs of H&E staining in each group. The scale bars are 100 µm in the original images and 20 µm in the enlarged images. d The statistical charts of activated and atretic follicles (values are mean ± SEM). The number of sections used for statistics: Con n = 15; 10−8 M MLT n = 12; 10−7 M MLT n = 9. e–g The effect of MLT addition on early follicle growth and atresia. n = 5 (Con), 4 (10−8 M MLT and 10−7 M MLT) biologically independent ovaries, respectively. e Experimental design. f Representative photographs of H&E staining. The scale bars are 200 µm in the original images and 80 µm in the enlarged images. g The statistical charts of follicles beyond type 5a-stage and atretic follicles (values are mean ± SEM). The number of sections used for statistics: Con n = 10, 10−8 M MLT n = 7, 10−7 M MLT n = 8. Statistical significance was determined using one-way ANOVA followed by Tukey’s post hoc test. Significant differences are denoted by *P < 0.05 and **P < 0.01.