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. 2021 May 6;11:9683. doi: 10.1038/s41598-021-89239-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Histological comparison of the inhibitory effects of cultured MSCs versus thawed cryopreserved MSCs on OA progression in both knees of the same rat. (A) Histological images for medial tibial and femoral condyles stained with safranin O. Best, representative, and worst images are selected based on Osteoarthritis Research Society International (OARSI) histological scores on the cultured cell side. (B) Statistical analysis of OARSI histological score. NS, not significant by Wilcoxon's signed rank test (n = 9).