Table 2.

Differentially expressed miRNAs in infantile-onset and adult-onset TLE.

Epilepsy onset	miRNA	Acute phase				Latent phase				Chronic phase
		miQPCR		NGS		miQPCR		NGS		miQPCR		NGS
		p-value	FC	p-value	FC	p-value	FC	p-value	FC	p-value	FC	p-value	FC
Adult	miR-132-3p	*	1.9	**	1.8	–	1.2	**	1.4	–	− 1.2	*	1.4
	miR-132-5p	–	1.1	**	1.6	–	1.0	–	1.2	*	2.5	**	1.3
	miR-142-3p	*	2.7	**	1.9	–	1.3	**	3.1	–	1.3	*	1.5
	miR-142-5p	**	18.5	**	1.8	–	− 1.8	**	2.7	**	2.8	*	1.6
	miR-146a-5p	–	− 1.2	–	1.2	*	3.0	**	2.3	*	1.9	**	2.1
	miR-155-5p	*	6.7	**	6.5	–	1.1	**	5.9	–	− 1.2	**	2.9
	miR-185-5p	–	− 1.6	–	− 1.1	*	− 1.5	*	− 1.5	–	− 1.7	–	− 1.1
	miR-205	–	− 1.5	*	− 1.7	–	− 1.5	–	1.0	*	− 2.5	*	− 2.2
	miR-212-3p	*	1.6	**	2.8	–	− 1.0	**	1.7	–	− 1.3	**	1.7
	miR-221-3p	–	− 1.4	**	− 1.5	–	− 1.1	–	− 1.2	*	− 1.5	*	− 1.3
	miR-339-3p	–	− 1.6	–	− 1.1	–	− 1.5	*	1.3	*	− 1.9	*	− 1.5
	miR-361-3p	**	− 1.7	**	− 1.4	–	1.1	–	− 1.2	–	− 1.3	–	− 1.1
	miR-7b	–	− 1.0	–	1.0	*	− 5.0	**	− 1.5	–	− 2.1	**	− 1.9
Infantile	miR-132-5p	*	4.1	**	2.2	–	− 1.5	–	1.2	–	3.4	–	− 1.0
	miR-205	**	− 2.7	**	− 1.7	–	− 7.4	–	1.1	–	1.1	–	− 1.0
	miR-221-3p	*	− 1.4	**	− 1.3	–	− 3.3	–	− 1.1	–	1.5	–	− 1.1
	miR-330-3p	*	− 2.3	**	− 1.5	–	− 1.1	–	− 1.0	–	1.4	–	− 1.0
	miR-3473	**	− 1.9	*	− 2.7	**	− 3.4	–	1.8	–	− 1.2	–	− 1.9
	miR-451-5p	–	− 1.2	–	− 1.0	NA	NA	*	− 1.4	*	− 1.8	*	− 1.6
	miR-6215	*	− 2.1	*	− 2.7	–	− 3.2	–	− 1.2	–	− 1.5	–	1.5
	miR-7a-2-3p	*	− 3.3	**	− 1.5	–	− 3.0	–	1.2	–	− 1.7	–	− 1.4

List of miRNAs with altered regulation (fold-change over 1.4) confirmed by miRNA sequencing (DESeq2) and miQPCR. 13 miRNAs differentially expressed in adulthood onset epilepsy (six in acute, three in latent and six in chronic stage) with one miRNA common to the acute and chronic stage and two for the latent and chronic stage. Eight miRNA in total altered in animals with epileptic status at P12: seven altered in the acute stage and one in the chronic stage.

**p-value < 0.01; *0.01 < p-value < 0.05; – p-value > 0.05; NA not identified, FC fold change.