TABLE 1.

Primers characteristics and PCR conditions used for the amplification of the rpb1 sequences.

Primer	Nucleotide sequence (5′–3′)	Position on the sequence	In combination with	Expected amplicon length	Ta°C	t elong.
RPB1-3F	GTC TTC GTG CAG TTT GGG A	727–745	RPB1-5R [1 μM]	≈1,410 bp	55	1 min 30 s
RPB1-4F	CTA GGC CTG ATT GGA TGA T	1,204–1,222	RPB1-5RN [500 nM]	≈870 bp	54	1 min
RPB1-4F1	GCT CGT CCT GAT TGG ATG A	1,203–1,221	RPB1-5R [1 μM]	≈935 bp	55	1 min
RPB1-5R	ACG ATT TGT TTT GGT ACC AT	2,119–2,138
RPB1-5RN	TTC ATC TCA TCA CCA TCA A	2,048–2,066

The position of primers is given based on sequence of Rhizoglomus intraradices HG316020.1. The final concentration of the reverse primers is provided for each combination, as well as the expected product size, the temperature of annealing (Ta), and the time of elongation (t elong).