Fig. 2.

CD25 expression on tumor-infiltrating lymphocytes. (a) Tumor-infiltrating lymphocytes were analyzed with flow cytometry. Tregs accounted for 49.8% of CD4⁺ T cells. (b) 71.5% of CD25 positive cells were CD3⁺CD4⁺Foxp3⁺ Tregs (c) Upper: Surface expression of CD25 was evaluated for NK cells, CD8⁺ T cells, CD4⁺Foxp3⁻ T cells, and CD4⁺Foxp3⁺ Tregs. Lower: CD4⁺Foxp3⁺ Tregs showed significantly higher mean fluorescence intensity (MFI) of CD25 (left) and CD25 positive percentage (right) (n = 4; one-way ANOVA followed by Tukey's test; ****, p < 0.0001).