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. 2021 Apr 23;12:609821. doi: 10.3389/fmicb.2021.609821

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Copyright © 2021 Wang, Dai, Liu, Yang, Hou, Ou, Ding, Ma, Chen, Li, Sun, Zheng, Zhang, Wubshet, Zaberezhny, Aliper, Tarasiuk, Pejsak, Liu, Zhang and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Selection of optimal reaction conditions. Analysis of optimal primer set (A,B) And reaction temperature (C,D) By fluorescence signals and naked eye observation. One representative result of three replicates is shown. (A) Shows the fluorescence signals of the five candidate primer sets. The fluorescence signals at each primer set are shown in a different color. (B) Shows the visual LAMP result of five candidate primer sets, numbers 1 to 6 represent primer set 1, set 2, set 3, set 4, set 5, and negative control, respectively. Positive reactions are shown by “+” and negative reactions are shown by “-”. (C) Shows the fluorescence signals of five different reaction temperatures. The fluorescence signals at each temperature are shown in a different color. (D) Shows the visual LAMP result of five different reaction temperature, number 1 to 6 represent 62°C, 63°C, 64°C, 65°C, 66°C and negative control, respectively. Positive reactions are shown by “+” and negative reactions are shown by “-”.