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. 2021 Apr 23;12:609821. doi: 10.3389/fmicb.2021.609821

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Copyright © 2021 Wang, Dai, Liu, Yang, Hou, Ou, Ding, Ma, Chen, Li, Sun, Zheng, Zhang, Wubshet, Zaberezhny, Aliper, Tarasiuk, Pejsak, Liu, Zhang and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Performance comparison of different dyes for visual LAMP assay. (A) The visual LAMP results of different dyes by detecting 10⁶ copies/μL plasmid pUC57-p72 under daylight. Neutral red: number 1 to 2, cresol red: number 3 to 4, HNB: number 5 to 6, calcein: number 7 to 8, SYBR green 1: number 9 to 10. Positive reactions are shown by “+” and negative reactions are shown by “-”. (B) Visualization under UV irradiation. (C) The comparison of the sensitivity of different dyes by detecting pUC57-p72 standards, number 1 to 8 represent 10⁶ copies/μL, 10⁵ copies/μL, 10⁴ copies/μL, 10³ copies/μL, 10² copies/μL, 10¹ copies/μL, 10⁰ copies/μL, and negative control, respectively. The corresponding dyes from top to bottom are cresol red, HNB, calcein, and SYBR green. Positive reactions are shown by “+” and negative reactions are shown by “-”.