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. 2021 Apr 23;12:609821. doi: 10.3389/fmicb.2021.609821

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Copyright © 2021 Wang, Dai, Liu, Yang, Hou, Ou, Ding, Ma, Chen, Li, Sun, Zheng, Zhang, Wubshet, Zaberezhny, Aliper, Tarasiuk, Pejsak, Liu, Zhang and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analytical sensitivity of Visual LAMP assay. (A) Comparative analytical sensitivity of visual LAMP (open circles) and the gold standard qPCR (closed circles) using plasmid standards. (B) The visual LAMP results of the plasmid standards, number 1 to 8 represent 10⁶ copies/μL, 10⁵ copies/μL, 10⁴ copies/μL, 10³ copies/μL, 10² copies/μL, 10¹ copies/μL, 10⁰ copies/μL, and negative control, respectively. Positive reactions are shown by “+” and negative reactions are shown by “-”. (C) Determining the visual LAMP products by agarose gel electrophoresis. From left to right: lane M, DNA Marker DL-2000 (Takara); lanes 1-8, different copy number subjected to visual LAMP assay (10⁶ copies/μL, 10⁵ copies/μL, 10⁴ copies/μL, 10³ copies/μL, 10² copies/μL, 10¹ copies/μL, 10⁰ copies/μL and negative control, respectively).