TABLE 1.
Visual LAMP primers used for detection of ASFV based on the conserved region of the p72 gene.
| Primer | Type | Positiona | Sequence (5′–3′)b |
| ASFV-LP-F3 | Forward outer | 895-914 | ACGGACGCAACGTATCTGGA |
| ASFV-LP-B3 | Reverse outer | 1153-1169 | TTGCGTCTACTGGGGCG |
| ASFV-LP-FIP (F1c+TTTT+F2) | Forward inner | 974-996, 909-933 | CTTAATCCAGAGCGCAAGAGGGGTTTTTCTGGACATAAGACGTAATGTTCAT |
| ASFV-LP-BIP (B1c+TTTT+B2) | Reverse inner | 1051-1070, 1092-1115 | CCCTTCGGCGAGCGCTTTATTTTTGGAAATTCATTCACCAAATCCTTT |
| ASFV-LP-FLOOP | Forward Loop | 951-974 | GGCTGATAGTATTTAGGGGTTTGA |
| ASFV-LP-BLOOP | Reverse Loop | 1068-1090 | TATCACCATAAAGCTTGCATCGC |
aPosition numbers are based on the complete p72 gene of ASFV SY18 strain (GenBank No. MH713612.1).bBold face indicates the TTTT spacer between F1c and F2 or B1c and B2.