TABLE 1.

Visual LAMP primers used for detection of ASFV based on the conserved region of the p72 gene.

Primer	Type	Positiona	Sequence (5′–3′)b
ASFV-LP-F3	Forward outer	895-914	ACGGACGCAACGTATCTGGA
ASFV-LP-B3	Reverse outer	1153-1169	TTGCGTCTACTGGGGCG
ASFV-LP-FIP (F1c+TTTT+F2)	Forward inner	974-996, 909-933	CTTAATCCAGAGCGCAAGAGGGGTTTTTCTGGACATAAGACGTAATGTTCAT
ASFV-LP-BIP (B1c+TTTT+B2)	Reverse inner	1051-1070, 1092-1115	CCCTTCGGCGAGCGCTTTATTTTTGGAAATTCATTCACCAAATCCTTT
ASFV-LP-FLOOP	Forward Loop	951-974	GGCTGATAGTATTTAGGGGTTTGA
ASFV-LP-BLOOP	Reverse Loop	1068-1090	TATCACCATAAAGCTTGCATCGC

^aPosition numbers are based on the complete p72 gene of ASFV SY18 strain (GenBank No. MH713612.1).^bBold face indicates the TTTT spacer between F1c and F2 or B1c and B2.