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. 2021 Apr 14;24(5):102424. doi: 10.1016/j.isci.2021.102424

Figure 5.

Figure 5

Partial tumor cell death induction accelerates Ti-DC migration

Tumors consisted of MC38/TfROVA-DTR and MC38 cells (mixed in the 3:7 ratio).

(A and B) Organs from CD11c-YFP mice bearing mixed tumors were analyzed 24 h after DT or vehicle treatment. Fluorescent microscopic images of tumors (A), number of Keima+CD45– cells (B). While arrows indicate YFP+ cells that phagocytosed dying tumor cells. Scale bar, 50 μm. Bar graph shows means ± SEM of pooled data from two independent experiments (n = 6−7). ∗∗∗p < 0.001 (Mann-Whitney U test).

(C) Number of KikGR-Red cells in MHC class IIhigh total migratory DCs and DC subsets in dLNs from vehicle or DT-treated KikGR mice bearing mixed tumors at indicated time points following photoconversion. Bar graphs show means ± SEM of pooled data from two independent experiments (n = 7−10). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. (Mann-Whitney U test).