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. 2021 Apr 14;24(5):102424. doi: 10.1016/j.isci.2021.102424

Figure 7.

Figure 7

Partial tumor cell death induction increases tumor antigen-specific effector T cells

(A) Experimental setup for the analysis of endogenous tumor-antigen specific T cells after partial tumor cell death induction. Mixture of MC38/TfROVA-DTR and MC38 cells (3:7 ratio) was intradermally inoculated into abdominal skin, and B16-OVA tumor cells were subcutaneously inoculated into flank skin of C57BL/6 mice 5 days before tumor cell death induction. Tumor cell death was induced at day 0. Two, five, and ten days later, endogenous tumor-antigen-specific T cells were analyzed.

(B) Number of OVA tetramer+ CD8+ T cells at indicated time points following DT or vehicle treatment. Line graphs show means ± SEM of pooled data from at least two independent experiments (n = 8−12). ∗p < 0.05 (Mann-Whitney U test).

(C) Number of DPEC, MPEC, and SLEC in OVA tetramer+ CD8+ T cells 2 days following vehicle or DT treatment. Bar graphs show means ± SEM of pooled data from two independent experiments (n = 8). As values of two samples of DEPCs in axillary LN from Vehicle group and 1 sample of MEPCs in Tumor from Vehicle and DT groups were zero, these data were not shown in these figures. ∗p < 0.05, ∗∗p < 0.01 (Mann-Whitney U test).