Figure 4.

A, addition of DC₁₂-CoA into liver homogenate generated succinate dose-dependently, as completely abolished by pretreatment with TDYA-CoA.B, DCA₁₂ addition elevated βOHB/AcAc ratio dose-dependently in liver homogenate. C and D, addition of DCA₁₂ into liver homogenate led to accumulation of 3-OH-CoA (C) and 2-enoyl-CoA (D) intermediates with hexanoate as a substrate, as reduced by pretreatment with TDYA-CoA or malonate. E, addition of DCA₁₂ into liver homogenates led to accumulation of hexanoyl-CoA (C6-CoA) with hexanoate as a substrate, as lowered by pretreatment with TDYA-CoA or malonate. Mean ± SEM, n = 6, ∗p < 0.05 by one-way ANOVA with Dunnett’s T3 test compared with the control or t-test between paired conditions.