Fig. 5.

Sequence Logos of the active sites of glutaredoxins present in vertebrates obtain from a multiple sequence alignment of 500 to 1000 glutaredoxins sequences. Active site described for each glutaredoxin. Consensus: most common amino acid found in the alignment. Sequence Logo representing the conservation at each position. Identity: Green: 100% identity, Greenish-brown: at least 30% and under 100% identity, Red: below 30% identity. Sequences logos were produced from multiple sequence alignments, where the height of the amino acid symbol represents its relative frequency and the height of the stack represents the sequence conservation at each position [233]. B) Representation of the tridimensional surface and secondary structure of human glutaredoxins evidencing the active site in yellow (Protein Data Base structures 1B4Q, 2CQ9, 2DIY and 2MMZ for GRX1, GRX2, GRX3 and GRX5, respectively) visualized using PyMOL 2.4. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)