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. 2021 Apr 23;9:634355. doi: 10.3389/fcell.2021.634355

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Copyright © 2021 Barbosa, Gomes, Sequeira, Gonçalves-Ribeiro, Pina, Carvalho, Moreira, Vaz, Vaz and Brites.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Proteomic analysis reveals that treatment of mSOD1 astrocytes with pre-miR-146a reverts MN dysfunction and microglia activation by paracrine mediators. Astrocytes were isolated from the cortex of SOD1-G93A (mSOD1) mice with 7-day-old and cultured for 13 days in vitro. Transfection with pre-miR-146a was performed. Cell-derived secretome was added to (A) WT NSC-34 motor neurons (MNs) for 48 h or (B) naïve N9 microglia for 24 h. MA plots (left panels) were obtained by comparison of the proteomic profiles of the treatment with the secretome of pre-miR-146a treated mSOD1 astrocytes and the untreated ones. Here, x axis is the mean log2 intensity (fold change) of each protein between two profiles (A-value) and y axis is the average expression (signal_sum) of protein intensities (M-value). Summary tables (right panels) show the obtained hits and their classification according to biological processes using PANTHER Classification System as indicated in methods.