Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 23;9:662903. doi: 10.3389/fcell.2021.662903

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Hwang, Nawaz, Choi, Wang, Hussain, Nawaz, Lopez-Giraldez, Jeong, Dong, Oh, Bilguvar, Mane, Lee, Bystroff, Lifton, Ahmad and Chung.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Formation of the basal body and annulus is deregulated in Dnah2^Δ/Δ epididymal sperm. (A,B) Confocal images of immunostained centriolar (A) and annulus (B) proteins in WT (left) and Dnah2^Δ/Δ (right) epididymal sperm. Centriolar proteins, CENTRIN1 (top) and γ-Tubulin (bottom), lacking in mouse epididymal sperm, are aberrantly detected near the connecting piece of Dnah2^Δ/Δ sperm (A). Annulus components, SEPTIN4 (top) and SEPTIN12 (bottom), is localized heterogeneously along the flagella in Dnah2^Δ/Δ sperm (B). Hoechst was used for counter staining. Merged fluorescence and corresponding DIC images are shown (A,B). Magnified insets are represented (scale bars = 1 μm). (C,D) Transmission electron microscopy of normal (C) and Dnah2^Δ/Δ (D) epididymal sperm. Dnah2^+/Δ (top) and WT (bottom) sperm were used for controls to show ultrastructure of connecting piece lacking centrioles and annulus, respectively (C). Centriole-like structure or the traces are detected (red asterisks) and annulus-like electron-dense area (yellow asterisks) is localized heterogeneously near the connecting piece in Dnah2^Δ/Δ sperm (D). Nu, nucleus; BP, basal plate; CP, capitulum; SC, segmented column; PCV, proximal centriolar vault; DCV, distal centriolar vault; AN, annulus; AX, axoneme; M, mitochondria; ODF, outer dense fiber; MP, midpiece; PP, principal piece.