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. 2021 Mar 23;13(5):e13412. doi: 10.15252/emmm.202013412

Figure 5. Analysis of T‐cell receptor repertoires in BrM and CLN.

Figure 5

  1. Experimental design of TCR sequencing.
  2. Percent T cells of estimated cells in BrM and CLN of mice from control or WBRT group.
  3. Maximal productive frequency of clones in BrM and CLN samples.
  4. Productive clonality in BrM and CLN samples.
  5. Clonal space homeostasis of BrM samples indicating the relative abundance of clones with a specific frequency.
  6. Lorenz curves generated with the reads of all clones in a sample to visualize deviation from perfect equality.
  7. Lorenz curves were used to determine the Gini index of each sample, which is a measure of inequality reflecting clonal expansion.
  8. Percent of estimated T cells correlated with BrM volume.(r = −0.7310, P = 0.0252).
  9. Gini indices of BrM samples correlated with BrM volume (r = −0.9453, P = 0.0001).

Data information: In (B–D + G), data are represented as scattered dot plot with line mean ± SD. P‐values were obtained by Mann–Whitney test for (C, D) and unpaired t‐test for (G) with **P < 0.01, and ***P < 0.001. For Control: n = 5 for BrM and CLN, for WBRT: n = 4 for BrM and n = 5 for CLN. In (H, I), the simple linear regression was plotted and Pearson’s correlation was used to obtain correlation coefficient r with P‐value. Exact P‐values can be found in Appendix Table S3.

Source data are available online for this figure.