FIGURE 1.

Common cell culture system. Monoculture is usually carried out directly on the rigid plastic culture substrate; A common use of co-culture is to grow both types of cells in the same extracellular fluid using Transwell chambers; The first two systems of culture were homogenous drug distribution when it comes to the treatment medium or the stimulation. The most commonly used 3D culture system is ECM embedded culture and static suspension culture. The neuro-spheroids or microscopic tissue model formed by 3D culture technology can better simulate the microenvironment in vivo, and the distribution gradient of drugs in vivo can be simulated more accurately when the treatment medium or stimulation is given.