Fig. 2.

TEP1 and CTL4 are required for defense against systemic Sm infections. a, b Survival assays of the indicated mosquito genotypes after injection with Sm (OD₆₀₀ = 0.0005). One representative experiment is shown from at least 3 independent biological experiments. The Kaplan-Meier survival test was used to calculate the percent survival. Statistical significance of the observed differences was calculated using the log-rank test. c, d Bacterial proliferation assays conducted on the indicated mosquito genotypes injected with Sm (OD₆₀₀ = 0.0005). Batches of 8 whole mosquitoes were grinded each in the LB medium at 24 h after infection, and CFUs were scored on LB plates supplemented with the appropriate antibiotic. Each circle on the scatter plot represents the mean CFU per mosquito per batch. Statistical analysis was performed using the Mann-Whitney test, and medians were considered significantly different if p < 0.05. Data shown are from 9 independent biological experiments. e Western blots showing the knockdown efficiencies of TEP1 and CTL4. αSRPN3 was used to control for loading. TEP1, thioester-containing protein 1; CTL4, C-type lectin 4; Sm, Serratia marcescens; CFU, colony-forming unit.