Fig. 3.

CTL4 and Tep1 are not required for defense against Sm oral infections. a Survival assays of the indicated mosquito genotypes after oral infection with Sm (OD₆₀₀ = 1). One representative experiment is shown from at least 3 independent biological experiments. The Kaplan-Meier survival test was used to calculate the percent survival. Statistical significance of the observed differences was calculated using the log-rank test. b Bacterial proliferation assays conducted on the indicated mosquito genotypes after oral infection with Sm (OD₆₀₀ = 1). Hemolymph was perfused from batches of 5 mosquitoes at 72 h after feeding on Sm, and CFUs were scored on LB plates supplemented with the appropriate antibiotic. Each circle on the scatter plot represents the mean CFU per mosquito per batch. Statistical analysis was performed using the Mann-Whitney test. Medians (black lines) were considered significantly different if p < 0.05. Data shown are from 3 independent biological experiments. TEP1, thioester-containing protein 1; CTL4, C-type lectin 4; Sm, Serratia marcescens; CFU, colony-forming unit.