Figure 5.

L5P used to discriminate MAP infected cattle from M. bovis infected cattle. ELISA were performed on plates coated with L5P in ethanol. The panel of sera tested included 3 MAP positive sera of reference, 3 negative controls, and 11 M. bovis positive sera. Serum samples were tested in triplicate. The statistical differences between the groups were determined using the non-parametric Mann–Whitney test. Significantly different when p < 0.05. ****p < 0.0001. Not significantly different (ns).