Dietary Mn supplementation increases tolerance in response to DSS treatment. A, Schematic of the DSS-induced colitis model. Mice were fed with either Mn-adequate (Mn-A) or Mn-supplemented (Mn-S) diets (35 and 300 mg/kg Mn, respectively). For colitis induction, mice were provided with drinking water containing 3% (w/v) DSS for 6 days (inflammatory phase). The mice were then placed on regular drinking water for 7 days (recovery phase). B, Body weight changes over time. Mice were treated with 3% DSS in drinking water for 6 days, followed by 7 days of regular drinking water. Body weight was measured over the entire 13 day period (n = 15 per group). C, Colon length from mice fed Mn-adequate (Mn-A) or Mn-supplemented (Mn-S) diets on Day 6 after DSS treatment (n = 4–5 per group). Data represent means ± SEM. *P < .05