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. 2021 Apr 23;12:635622. doi: 10.3389/fimmu.2021.635622

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Copyright © 2021 Anisuzzaman, Frahm, Prodjinotho, Bhattacharjee, Verschoor and Prazeres da Costa

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mouse serum factors can partially antagonize survival promoting effects of human serum. (A) NTS were cultured and maintained in the presence of 10% of mouse (MSe) or human (HSe) sera or adding both at 10-20% and scoring was performed at the indicated time points. Hybridoma medium (HM) alone was used as a control. Each data point has been shown as mean ± SD of at least three technical replicates. (B) Morphological changes of NTS in presence of 10% MSe, HSe or in combination (10-20%). Representative photographs of NTS were taken using a digital camera fitted with an inverted microscope (10x). Arrow indicates development of vacuoles. p.t., post transformation; s.p., scoring point.