(A) Migration velocity of pDFs from WT and cPLA2α mice treated with ethanol or the indicated concentrations of PGE2, 5-HETE, or 20-HETE. (B) Migration velocity of pDFs from KO mice treated with increasing concentrations of 5-HETE. (C–E) Migration velocity of pDFs from WT, KI, and KO mice in the presence of pyrrophenone (C), MK886 (D), or the indicated combinations of pyrrophenone, MK886, and 5-HETE (E). Samples were compared using ANOVA followed by Tukey’s post-hoc test. Data shown are means ± SD, n = 3–10 cell isolates per genotype (3–8 mice per genotype were used to generate the cell isolates) for each treatment group. *P< 0.05, **P< 0.01, ***P< 0.001, ****P< 0.0001.