Table 1.
Primers used in this study.
| Primer Name | Sequence | Use in This Study |
|---|---|---|
| CgEnd3-5Ffor (1F) | AGACTGGCCAAAAGTGTTCG | 5F flanking sequence |
| CgEnd3-5Frev (2R) | CTCCCACAGGAATCTCCGTC | |
| CgEnd3-3Ffor (2F) | TACGCTTCGATACCCTTCGG | 3F flanking sequence |
| CgEnd3-3Frev (3R) | CTTGGAGCGACAAGTTGGGA | |
| External-CgEnd3for (3F) | TTCCTAGCGACCCTGTTGTT | External sequence used for validation of mutant |
| External-CgEnd3rev (4R) | GCCCGGTGAGTAGAATGGTA | |
| Internal-CgEnd3for (4F) | AATAACAACCCCGCCTCTTC | Internal sequence used for validation of mutant |
| Internal-CgEnd3rev (5R) | AGCTGCTTCTTGAGCCTGAC | |
| CgEnd3-Compfor | TCGACTCGACAACATCAAGC | Complementary sequence |
| CgEnd3-Comprev | GCCCGGTGAGTAGAATGGTA | |
| SUR-5′-M13F | CGCCAGGGTTTTCCCAGTCACGACGTCGACGTGCCAACGCCACAG | Sur cassette contains the region of M13F and M13R |
| SUR-3′-M13R | AGCGGATAACAATTTCACACAGGAGTCGACGTGAGAGCATGCAAT | |
| SU-SPLIT | CCAAGCATGTGCAGTGCCTTC | The 2/3rd portion of the Sur cassette |
| UR-SPLIT | GGAGGCCGACGTCATAGGCATC | |
| CgPMC1-f | CATCATGATTGCTGGTCAGG | qRT-PCR of CgPMC1 |
| CgPMC1-r | GACACCGAAAGGAATGGAAA | |
| CgNCS1-f | AGCGACAAGTCAGGAAGCAT | qRT-PCR of CgNCS1 |
| CgNCS1-r | CCTCGACGATCTTGAGCATT | |
| CgCAM1-f | ACAACAACGGCTTCATCTCC | qRT-PCR of CgCAM1 |
| CgCAM1-r | GCGAATCATCTCGTCAACCT | |
| CgCRZ1-f | AGGTCGGATCTGCATCAAAC | qRT-PCR of CgCRZ1 |
| CgCRZ1-r | ATGTACTGGCCGCTGGTATC | |
| CgCAMK1-f | ATGCTGAAGAAGGGTCATGG | qRT-PCR of CgCAMK1 |
| CgCAMK1-r | TGAAATCCTTGGCATCATCA | |
| CgCHS1-f | GGTGGTGGTCTGAAGCGTTA | qRT-PCR of CgCHS1 |
| CgCHS1-r | AGCGCATCTTGTGGAACTCA | |
| CgCHS2-f | GACTACGCCCGCGAATATGA | qRT-PCR of CgCHS2 |
| CgCHS2-r | GTTCGTAGATGCCGGAAGGA | |
| CgCHS3-f | CACCGGCTACAGCGAGTATG | qRT-PCR of CgCHS3 |
| CgCHS3-r | CAAGTTTCCGCGGTACAGGA | |
| CgCHS4-f | CGACAAGGACCATCCGAACT | qRT-PCR of CgCHS4 |
| CgCHS4-r | GCGAGGCATCGGAGTAGTTA | |
| CgCHS5-f | CTCAGGGCGGCATTGATACT | qRT-PCR of CgCHS5 |
| CgCHS5-r | AGTAGAGCGTAGTTGGAGGC | |
| CgCHS6-f | ACCAATCCGGTGCTTATCGG | qRT-PCR of CgCHS6 |
| CgCHS6-r | GGGGTCTCGAAGCCAAGATG | |
| CgCHS7-f | TGGTCAAGGGCCTTCAATGG | qRT-PCR of CgCHS7 |
| CgCHS7-r | ATCACAACCTTTGGTGCGGA | |
| CgDN3-f | CCTACTCGCTGTTCCCTTCA | qRT-PCR of CgDN3 |
| CgDN3-r | CGTGGTCTCCCGGATAGTAG | |
| CgEC6-f | TTGGCAGTATCACCGTGAAG | qRT-PCR of CgEC6 |
| CgEC6-r | TCGATCTCATCCTGGTAGGC | |
| CgFl-f | GCTGTTGAGTCCGGTGGTAT | qRT-PCR of CgFl |
| CgFl-r | GGGTGGTGGTCATAGAGGTG | |
| CgNis1-f | ATCTACGGCATTGCCTTCC | qRT-PCR of CgNis1 |
| CgNis1-r | AGAAGGAGCCGATGACTGTG | |
| Cg18S-f | GTGAGGCCCTCAAAGGTAGTGG | qRT-PCR of Cg18S |
| Cg18S-r | GGATCCCAGTGCGAGACGT |