A, Flow cytometry panels depicting the gating strategy to identify and phenotype extravascular, host polyclonal and OT-I CD8+ T cells in grafts of F1.OVA + OT-I group. Minimal number of donor cells were detected in the graft. Representative of 5 mice at week 8 and 4 mice at week 4. B, Proportion of polyclonal and OT-I T cells that acquired a TRM phenotype in the same grafts at week 4 (n = 4, N = 2) and week 8 (n = 5, N = 2). Individual biological replicates and mean displayed.