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. 2021 May 4;218(7):e20201793. doi: 10.1084/jem.20201793

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© 2021 Zhou et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S3. — Schematic overview and flow cytometry plots showing the CFSE-based assay for tracking monocyte changes in response to peanut protein for PA subjects. (A) Schematic overview of the CFSE-based monocyte tracking assay. (B) Representative flow cytometry plots showing the purity of isolated untouched monocytes using Miltenyi pan monocytes isolation kit. (C) Representative flow cytometry plots show the CFSE⁺CD209⁺CD11c⁺ DCs in the mixed cells by combining CFSE-labeled monocytes and unlabeled autologous nonmonocytes before incubation with peanut proteins. (D) Flow cytometry plots gated on Lin (CD3, CD19, CD56) negative cells show the change of CFSE⁺ monocytes and CFSE⁺CD209⁺CD11c⁺ DCs by peanut protein from the PBMCs stimulated with or without peanut protein in seven PA participants and three healthy buffy coats.