Table 4.
Johne data
| Animal # | ELISA+/− | IFNγ+/− | Fecal PCR+/− | Phage/qPCR +/− |
|---|---|---|---|---|
| 1j | − | + | + | + |
| 2j | − | + | − | + |
| 3j | − | + | + | − |
| 4j | − | − | − | + |
| 5j | − | − | + | − |
| 6j | − | − | − | + |
| 7j | − | + | − | + |
| 8j | − | + | − | + |
| 9j | − | + | + | + |
| 10j | + | + | + | + |
| 11j | − | + | + | − |
| 12j | − | + | + | − |
| 13j | − | + | − | + |
| 14j | − | + | + | + |
| 15j | − | − | + | + |
| 16j | − | + | + | − |
| 17j | − | + | + | + |
| 18j | − | + | + | + |
| 19j | + | − | + | − |
| 20j | − | + | + | − |
| 21j | + | + | + | + |
| 22j | + | − | + | − |
| 23j | − | + | + | + |
| 24j | − | + | + | + |
| 25j | − | + | − | + |
| 26j | + | − | + | + |
| 27j | − | − | + | + |
| 28j | + | − | + | − |
| 29j | + | − | − | + |
| 30j | + | + | + | + |
| 31j | + | + | + | + |
| 32j | + | + | + | + |
| % positive (# positive/total tested) | 31% (10/32) | 69% (22/32) | 75% (24/32) | 72% (23/32) |
ELISA is the least sensitive at demonstrating MAP infection. IFN-γ, Fecal PCR and phage/qPCR have comparable sensitivity. Of the ten animals that were positive on ELISA, 5 were positive by all four methods. Two of the remaining ELISA positives were positive on Fecal PCR