Table 5.
Age, plaque numbers and qPCR (+/− IS900) for each animal
| Control | Johne | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| # | Age (years) | Plaque # | qPCR Pos/Neg | Total assay # (# Pos) | # | Age (years) | Plaque # | qPCR Pos/Neg | Total assay# (# Pos) |
| 1c | 8 | 11cal | + | 4 (1) | 1j | 8 | 27 | + | 7 (6) |
| 2c | 8 | 1000est | + | 9 (4) | 2j | 12 | 312 | + | 5 (1) |
| 3c | 12 | 3 | − | 4 (0) | 3j | 12 | 139 | − | 8 (0) |
| 4c | 12 | 238 | + | 4 (1) | 4j | 9 | 1000est | + | 4 (2) |
| 5c | 5 | 688 | + | 8 (7) | 5j | 9 | 25 | − | 8 (0) |
| 6c | 5 | 29 | + | 11 (3) | 6j | 9 | 30 | + | 12 (2) |
| 7c | 4 | 10 | − | 4 (0) | 7j | 5 | 189 | + | 4 (2) |
| 8c | 4 | 492 | + | 16 (3) | 8j | 5 | 98 | + | 12 (3) |
| 9c | 3 | 1 | − | 4 (0) | 9j | 13 | 14 | + | 4 (1) |
| 10c | 3 | 151 | − | 5 (0) | 10j | 4 | 5 | + | 4 (1) |
| 11c | 3 | 47 | − | 4 (0) | 11j | 4 | 8 | − | 8 (0) |
| 12c | 1.3 | 4 | − | 4 (0) | 12j | 4 | 545 | − | 5 (0) |
| 13c | 2 | 1cal | No DNA | No DNA | 13j | 4 | 129 | + | 4 (2) |
| 14c | 2 | 8cal | − | 4 (0) | 14j | 3 | 78 | + | 12 (1) |
| 15c | 2 | 1cal | No DNA | No DNA | 15j | 3 | 38cal | + | 1 (1) |
| 16c | 2 | 4 | − | 4 (0) | 16j | 3 | 59 | − | 12 (0) |
| 17c | 1.3 | 6 | − | 4 (0) | 17j | 3 | 26 | + | 4 (1) |
| 18j | 3 | 214 | + | 5 (1) | |||||
| 19j | >11 | 48 | − | 12 (0) | |||||
| 20j | 7 | 449 | − | 3 (0) | |||||
| 21j | 5 | 91 | + | 14 (7) | |||||
| 22j | 5 | 135 | − | 2 (0) | |||||
| 23j | 5 | 308 | + | 15 (1) | |||||
| 24j | 5 | 321 | + | 14 (3) | |||||
| 25j | 4 | 97 | + | 11 (3) | |||||
| 26j | 11 | 145 | + | 4 (4) | |||||
| 27j | 10 | 1000est | + | 8 (5) | |||||
| 28j | 5 | 359 | − | 2 (0) | |||||
| 29j | 9 | 229 | + | 12 (3) | |||||
| 30j | 7 | 17 | + | 4 (2) | |||||
| 31j | 5 | 64 | + | 12 (3) | |||||
| 32j | 3 | 5 | + | 4 (4) | |||||
| Mean = 159±71 | += 35% (6/17) | Mean = 194±44 | += 72% (23/32) | ||||||
The count number of plaques (mean ± SEM) presented for 10% of the PBMC’s harvested from 8 ml whole blood following Ficoll gradient. When no plaques were seen, the number presented as “cal” (see Materials and methods). The number of individual qPCR replicates are presented as the total for a given animal with the number of positives in adjacent parentheses (see Materials and methods). “No DNA” indicates that there were so few plaques, that no DNA was extracted. In the Control animals, MAP + animals had significantly more plaques than MAP- animals (Fig 3)