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. 2021 May 6;19:96. doi: 10.1186/s12915-021-01033-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Comparison of tolerance to drought stress, population differentiation, and nucleotide diversity ratio among different M. ruthenica accessions. a The geographic sites for collection of 20 M. ruthenica accessions. b Survival rates of 20 M. ruthenica accessions at the twelfth day exposed to drought stress. Four biological replications and 15 seedlings in each biological replication were used to determine survival rate. All the survival rates under control are 100%. Different letters mean significant differences among treatments at P<0.05. Data are means±SE (n=4). c Correlations between survival rates under drought stress and annual precipitation of sites at which the M. ruthenica seeds were collected. d Phenotypes of two accessions collected from sites of Zhenglanqi and Xinghe. The phenotypes were photographed at the twelfth day of drought. e Neighbor-joining tree of M. ruthenica accessions. Mt J.A17: M. truncatula Jemolong A17. f Population differentiation (Fst). g Nucleotide diversity ratio (log₂(θπ_Control/θπ_Tolerance)) between tolerance and control population. Points above lines are 5% of the biggest Fst and log₂(θπ_Control/θπ_Tolerance), respectively. Points of log₂(θπ_Control/θπ_Tolerance) less than zero are not shown