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. 2021 May 6;19:95. doi: 10.1186/s12915-021-01030-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — TORC1 regulates the expression of glucose-responsive genes independently of Sch9. Wild type and sch9Δ cells were grown to logarithmic phase in SC/EG medium and then glucose (2% final concentration) was added to the cultures in the presence of either rapamycin (200 nM) or DMSO. Aliquots of the cultures were taken after 0’, 30’, 60’, and 120’. RNA was extracted from the cultures and the expression of the indicated 7 TGC genes (GFD2, GPG1, UGA1, RME1, CIT1, CRC1, and DHR2) were analyzed by real-time qRT-PCR. Data are presented as means ± standard deviation (n = 2 technical replicates). Data from two additional biological replicates of this experiment are presented in Fig. S7