Fig. 6.

Regulation of glucose-responsive genes by TORC1 is dependent on Tap42. Wild type or tap42-11 cells were grown to logarithmic phase at 25 °C (permissive temperature) in SC/EG medium and then shifted to 37 °C for 30’ to inactivate tap42-11. Glucose (2% final concentration) was added to the cultures in the presence of either rapamycin (200 nM) or DMSO. Aliquots of the cultures were taken after 0’, 30’, 60’, and 120’. RNA was extracted from the cultures and the expression of the 7 TGC genes (GFD2, GPG1, UGA1, RME1, CIT1, CRC1, and DHR2) were analyzed by real-time qRT-PCR. Data are presented as means ± standard deviation (n = 2 technical replicates). Data from two additional biological replicates of this experiment are presented in Fig. S9