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. 2021 Mar 25;95(8):e02296-20. doi: 10.1128/JVI.02296-20

FIG 3.

FIG 3

circPIKfyve regulates miR-21-3p expression and activity. (A) The Ago2-RIP assay for the amount of circPIKfyve in MIC transfected with Ago2-flag or pcDNA3.1-flag. We also detected the experimental group’s Western blot of Ago2 protein in input, anti-Flag, and IgG. (B) A schematic illustration showing overlapping of the target miRNAs of circPIKfyve predicted by TargetScan, miRanda, and MicroInspector. (C and D) Relative expression of candidate miRNAs in MIC and MKC transfected with si-circ (C) and oe-circ (D), respectively. (E) circPIKfyve reduces miR-21-3p activity. The relative luciferase activity was analyzed in MKC cotransfected with mimics, circPIKfyve overexpression plasmid, and control vector, together with miR-21-3p sensor. All data represent the means ± SE from three independent triplicate experiments. *, P < 0.05; **, P < 0.01.