FIG 6.

circPIKfyve acts as a sponge of miR-21-3p to enhance MAVS expression. (A and B) Relative mRNA and protein levels of MAVS in MIC or MKC after cotransfection with NC, si-circ, vector, or oe-circ by Western blotting (A) and qPCR assays (B). (C) Relative mRNA levels of MAVS in MIC or MKC after cotransfection with NC, si-circ, vector, or oe-circ by qPCR assay. (D) The relative luciferase activities were detected in EPC after cotransfection with MAVS-3′UTR luciferase reporter vector, NC, mimics, oe-circ, or oe-circ-mut. (E) Western blot assays were performed in MKC after cotransfection with MAVS overexpression plasmid, NC, miR-21-3p mimics, oe-circ, or oe-circ-mut. (F) oe-circ counteracts the negative effect of miR-21-3p. Relative luciferase activities were detected in MIC after cotransfection with MAVS expression plasmid, pRL-TK Renilla luciferase plasmid, luciferase reporters, NC, mimics, or oe-circ. (G) Cell proliferation was assessed by EdU assays in MKC after cotransfection with NC, mimics, or oe-circ. All data represent the means ± SE from three independent triplicate experiments. *, P < 0.05; **, P < 0.01.