FIG 7.

circPIKfyve is regulated by the RNA-binding protein QKI. (A) Schematic diagram of QKI promoting circPIKfyve formation. (B) qPCR analysis of QKI mRNA in spleen cells treated with SCRV (MOI, 5) at the indicated time points. (C) qPCR analysis of QKI mRNA in MIC treated with si-QKI. (D) si-QKI regulates the mRNA expression of circPIKfyve upon SCRV infection. MIC were transfected with si-NC or si-QKI for 48 h and then treated with SCRV. The expression of circPIKfyve and linear circPIKfyve was analyzed by qPCR assays. All data represent the means ± SE from three independent triplicate experiments. *, P < 0.05; **, P < 0.01.