FIG 8.

ceRNA network of regulating MAVS is widely found in teleost fish. (A) Sequence alignment of circPIKfyve from various teleost fish species. (B) The relative luciferase activities were detected in EPC after cotransfection with LcrcircPIKfyve-wt or LcrcircPIKfyve-mut and mimics or NC (left) and EPC after cotransfection with NdicircPIKfyve-wt or NdicircPIKfyve-mut and mimics or NC (right). (C) LcrcircPIKfyve and NdicircPIKfyve reduce miR-21-3p activity. EPC were transfected with LcrcircPIKfyve or NdicircPIKfyve expression plasmid, mimics, and control vector, together with the miR-21-3p sensor. The luciferase activity was analyzed and normalized to Renilla luciferase activity. (D) Sequence alignment of MAVS containing miR-21-3p binding site among different teleost species. (E and F) miR-21-3p regulating the luciferase activity of MAVS-3′UTR is examined in N. diacanthus and L. crocea. All data represent the means ± SE from three independent triplicate experiments. **, P < 0.01.