TABLE 1.
PCR primer information in this study
| Primer | Sequence (5′–3′) |
|---|---|
| MAVS-qRT-F | AGGCACCAACAATTCCAG |
| MAVS-qRT-R | ACGGAGCAGGCTTCACTT |
| TNF-α-qRT-F | GTTTGCTTGGTACTGGAATGG |
| TNF-α-qRT-R | TGTGGGATGATGATCTGGTTG |
| IL-1β-qRT-F | TACGATGGCTAATAACTCC |
| IL-1β-qRT-R | CATTGACAAAGTGCTCCA |
| MX1-qRT-F | GCTGCTTGTTTACTCCCA |
| MX1-qRT-R | ACCTGCATCATCTCCCTC |
| ISG15-qRT-F | TGAACGGACAGAAGACGC |
| ISG15-qRT-R | TGAGGAATACCTGCATGG |
| circPIKfyve-divergent-F | TACGCCCACTCAGCTGAT |
| circPIKfyve-divergent-R | TTTCCTGCTGCCGTCTCT |
| circPIKfyve-convergent-F | ACAGAACCCAAAATTGCC |
| circPIKfyve-convergent-R | AACATCCCCGTATCATCC |
| miR-21-3p-qRT-F | CGACAACAGTCTGTAGGCT |
| miR-21-3p-qRT-R | GGTCCAGTTTTTTTTTTTTTTTCAATG |
| miR-214-5p-qRT-F | GCCTGTCTACACTTGCTG |
| miR-214-5p-qRT-R | GTCCAGTTTTTTTTTTTTTTTGCAC |
| miR-143-5p-qRT-F | TGCAGTGCTGCATCTC |
| miR-143-5p-qRT-R | GTCCAGTTTTTTTTTTTTTTTCCAGA |
| miR-30c-3p-qRT-F | GCTGGGAGAGGGGTGT |
| miR-30c-3p-qRT-R | TCCAGTTTTTTTTTTTTTTTAGCGT |
| miR-103-5p-qRT-F | GAGCCTCTTTACAGTGCTG |
| miR-103-5p-qRT-R | GGTCCAGTTTTTTTTTTTTTTTCAAG |
| 5.8S rRNA-qRT-F | AACTCTTAGCGGTGGATCA |
| 5.8S rRNA-qRT-R | GTTTTTTTTTTTTTTTGCCGAGTG |
| GAPDH-qRT-F | ACCTTCACTCCTCCATCTT |
| GAPDH-qRT-R | AGGTCACAGACACGGTTG |
| U6-qRT-F | TGCGAGTAGCAGACCA |
| U6-qRT-R | CACGAGACCGAAACAC |
| circPIKfyve-F | CGGAATTCTAATACTTTCAGACGGCAGCAGGAAATCAG |
| circPIKfyve-R | CGGGATCCAGTTGTTCTTACCTCTGCCAGGTCAGGTCTTC |
| circPIKfyve-pmirGLO-F | CGAGCTCACGGCAGCAGGAAATCAG |
| circPIKfyve-pmirGLO-R | TGCTCTAGACTCTGCCAGGTCAGGTCTTC |
| circPIKfyve-pmirGLO-mut-F | TGAGCGCCCGTCTTGACTCGCCCTGCTCTGTGT |
| circPIKfyve-pmirGLO-mut-R | AGTCAAGACGGGCGCTCAGGTCCTCTCCGATGG |
| circPIKfyve-mVenus-F | TCAGATCTCGAGCTCAAGCTTACGGCAGCAGGAAATCAGAC |
| circPIKfyve-mVenus-R | CGGGCCCGCGGTACCGTCGACCTCTGCCAGGTCAGGTCTTCC |
| circPIKfyve-mVenus-mut-F | TGAGCGCCCGTCTTGACTCGCCCTGCTCTGTGT |
| circPIKfyve-mVenus-mut-R | AGTCAAGACGGGCGCTCAGGTCCTCTCCGATGG |
| miR-21-3p sensor-F | TCGAGGCTGTTGTCAGACATCCGACAGGCTGTTGTCAGACATCCGACAGGC |
| miR-21-3p sensor-R | GGCCGCCTGTCGGATGTCTGACAACAGCCTGTCGGATGTCTGACAACAGCC |
| Ago2-Flag-F | CCCAAGCTTGACAAAATGTATTCCTCTGC |
| Ago2-Flag-R | CGCGGATCCTTTCATCAGTGGGGTCTC |
| circPIKfyve-T7-F | TAATACGACTCACTATAGGGGCAGCCGCTGCTGCAACC |
| circPIKfyve-T7-R | AGAAGATCTGGCCGCACAGTC |
| pcDNA3.1-MS2-circPIKfyve-F | ACTATAGGGAGACCCAAGCTTGCAGCCGCTGCTGCAACC |
| pcDNA3.1-MS2-circPIKfyve-R | GCGGCCGTTACTAGTGGATCCAGAAGATCTGGCCGCACAGTC |
| pcDNA3.1-MS2-circPIKfyve-mut-F | TGAGCGCCCGTCTTGACTCGCCCTGCTCTGTGT |
| pcDNA3.1-MS2-circPIKfyve-mut-R | AGTCAAGACGGGCGCTCAGGTCCTCTCCGATGG |
| MAVS-HindIII-F | GACGATGACGACAAGAAGCTTTCGTCTGCCAAAGACAAACTGTA |
| MAVS-EcoRI-R | TGATGGATATCTGCAGAATTCCAGCCTCTGTCCTGTCTACTTCATG |
| MAVS-3′UTR-F | CTAGCTAGCCGTATGGTGCCTTATTG |
| MAVS-3′UTR-R | TGCTCTAGACAGCCTCTGTCCTGTCTACT |
| MAVS-3′UTR-mut-F | CACGAATGAAGATCAGACTGATGACTGTCTGCA |
| MAVS-3′UTR-mut-R | GTCTGATCTTCATTCGTGTACAAACATCTGTTATTATTATCATCATTATTATT |
| LcrMAVS-3′UTR-F | CCGCTCGAGACCCTCCAGACCTTTGAT |
| LcrMAVS-3′UTR-R | GTCGACGTCGACGCTCCTCGTTAATCCTCA |
| LcrMAVS-3′UTR-mut-F | ATCGCATTGAAGATCAGACTGATGACTCGTGC |
| LcrMAVS-3′UTR-mut-R | CTGATCTTCAATGCGATTATAAACACGTCTATTATTATCATCTTTATTATTG |
| NdiMAVS-3′UTR-F | AGAGGAACTTGGTTAGGTACCGGGGCTTTAAAACCTTTAAAGGA |
| NdiMAVS-3′UTR-R | CAGGTCGACTCTAGACTCGAGTCATTTATTTATAATTTCAGGTTTATTGATG |
| NdiMAVS-3′UTR-mut-F | AGACGCTACACGTGAAGATCAGACTGATGACTGTCATG |
| NdiMAVS-3′UTR-mut-R | CTTCACGTGTAGCGTCTATTATTATTATTATCATCATTATTATTAACA |
| LcrcircPIKfyve-F | CGGAATTCTAATACTTTCAGACGGCAGCAGGAAATCAG |
| LcrcircPIKfyve-R | CGGGATCCAGTTGTTCTTACCTCTGCCAGGTCAGGTCTTC |
| LcrcircPIKfyve-pmirGLO-F | CGAGCTCACGGCAGCAGGAAATCAG |
| LcrcircPIKfyve-pmirGLO-R | TGCTCTAGACTCTGCCAGGTCAGGTCTTC |
| LcrcircPIKfyve-pmirGLO-mut-F | TGAGCGCCCGTCTTGACTCGCCCTGCTCTGTGT |
| LcrcircPIKfyve-pmirGLO-mut-R | AGTCAAGACGGGCGCTCAGGTCCTCTCCGATGG |
| NdicircPIKfyve-F | CGGAATTCTAATACTTTCAGACGGCAGCAGGAAATCAG |
| NdicircPIKfyve-R | CGGGATCCAGTTGTTCTTACCTCTGCCAGGTCAGGTCTTC |
| NdicircPIKfyve-pmirGLO-F | CGAGCTCACGGCAGCAGGAAATCAG |
| NdicircPIKfyve-pmirGLO-R | TGCTCTAGACTCTGCCAGGTCAGGTCTTC |
| NdicircPIKfyve-pmirGLO-mut-F | TGAGCGCCCGTCTTGACTCGCCCTGCTCTGTGT |
| NdicircPIKfyve-pmirGLO-mut-R | AGTCAAGACGGGCGCTCAGGTCCTCTCCGATGG |