FIG 6.

Inhibition of LCMV infection by bergamottin and casticin. Vero cells were treated in duplicate with bergamottin (A) and casticin (B), respectively. After 1 h, cells were infected with LCMV (MOI, 0.01) for 1 h. After 23 h, the inhibition is evaluated using qPCR with the primers for (5′-AGAATCCAGGTGGTTATTGCC-3′) and rev (5′-GTTGTAGTCAATTAGTCGCAGC-3′). Bergamottin (C) and casticin (D) inhibited LCMV GPC-mediated membrane fusion. 293T cells were transfected with pCAGGS-LCMV GPC; 24 h later, compounds or vehicle (DMSO) was added for 1 h followed by treatment with acidified (pH 5.0) DMEM for 15 min. The cells were then placed in neutral-pH DMEM. Syncytium formation was visualized 1 h later using fluorescent microcopy. Images are representative fields from 3 independent experiments.