FIG 2.

M50-mediated IRE1 degradation is blocked by the p97/VCP inhibitor CB-5083. (A) MEFs were transfected with plasmids expressing Myc-tagged IRE1 and FLAG-tagged full-length or truncated (1–276) M50 or an empty vector (EV). Cells were treated with 30 μM CB-5083 or DMSO for the last 6 h before harvesting of lysates at 48 h posttransfection. (B) MEFs were infected with MCMV-GFP (MOI = 3). Cells were treated with 30 μM CB-5083 or DMSO for the last 6 h before harvesting. Cell lysates were analyzed by immunoblotting. M57 was detected as an infection control.