FIG 4.

Generation of SEL1L-deficient MEFs. (A) Sel1L knockout (ko) MEFs were generated by CRISPR/Cas9 gene editing. Two independent clones obtained with different gRNAs are shown. SEL1L and IRE1 protein levels were analyzed by immunoblotting. (B) Total RNA was isolated from WT and Sel1L knockout MEFs. mRNA levels of Ire1, Chop, Xbp1s, and Xbp1u were determined by qRT-PCR. Gapdh was used for normalization. Fold changes (Sel1L knockout compared to WT MEFs) are shown as means ± standard errors of the means (SEM) from three biological replicates. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.