FIG 6.

STAT6 does not bind the N4/N5 promoter of RTA/Orf50 in HE2.1 B cells. (A) HE2.1 B cells were treated with different concentrations of IL-4 for 24 h. Total and phosphorylated STAT6 proteins were determined by Western blotting. (B) A20 B cells were transfected with vectors expressing luciferase under the control of three different RTA/Orf50 promoters, termed proximal, distal, and N4/N5. A dual-luciferase reporter assay was performed 24 h after treatment with IL-4. (C) HE2.1 B cells were treated with different concentrations of IL-4 for 24 h. DNA bound to STAT6 was immunoprecipitated, and quantitative PCR (qPCR) was performed for the N4/N5 promoter region or arginase-1 (Arg1). The percentage of the input after normalization to the immunoglobulin G control was calculated for both N4/N5 and Arg1. Data are from three independent experiments. Error bars are standard errors of the means. P values were calculated by one-way ANOVA. **, P < 0.01; ****, P < 0.001.