FIG 4.

Serial passage lineage 1 to 3 reovirus segment profiles. RNA extracted from serial passage lysates was used as the template in RT-PCRs, along with primers that bind to the 3′- and 5′-terminal sequences of reovirus genome segments L1, M1, and S4. Products from reactions in which template RNA was extracted from rsT1L reovirus lin2 or lin3 P1 to P10 (A) or rsT3D^I reovirus lin2 or lin3 P1 to 10 (B) were resolved on 1.2% ethidium bromide-stained agarose gels. Gels from lin1 are identical to those shown in Fig. 2 but are included for comparison. Black asterisks indicate the position of the full-length segment. Filled triangles indicate products smaller than the full-length segment. Open triangles indicate products larger than the full-length segment.