FIG 4.

Barcodes shared between homotypic lineages at passage 10. (A) Histograms of the number of barcodes found in multiple (>1) lineages. Only the top 10 most frequent barcodes from each of the five lineages were analyzed (maximum of 50 barcodes). No barcodes were shared by multiple serial mosquito or alternating passage lineages, while 10 barcodes were shared by at least two serial mouse lineages. (B) Pairwise ranks for each of the 10 barcodes shared by more than one serial mouse lineage at passage 10. Colored symbols represent the 10 specific barcode sequences. The solid black line represents the line of best fit for all pairwise ranks. The nonparametric Spearman’s correlation coefficient (rs) and P value for all pairwise ranks are provided.