FIG 5.

Trajectories of individual single-nucleotide variants (SNVs) over sequential passages detected at >1% frequency in any passage. (A to C) SNV frequencies for individual SNVs detected in serial mouse lineages (A), serial mosquito lineages (B), and alternating passage lineages (C). Four nonsynonymous SNVs detected in all five mouse lineages are demarcated with arrows, and one SNV of note is similarly demarcated in alternating passage lineage A. Colors represent the same SNVs across homotypic lineages but are used more than once due to the large number of SNVs. The same SNVs may not be represented by the same colors across passage types. In the alternating passage series, odd passages are mouse sera and even passages are mosquito bodies.