Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Replication of SARS-CoV-2 in genetically modified human cell lines. Caco-2 and A549 cells were transduced or not with lentiviral vectors to stably overexpress either ACE2 or ACE2 together with TMPRSS2. The indicated (unmodified and modified) cell lines were infected with SARS-CoV-2 at MOI 0.05 and lysed 48 h later for RNA extraction and RdRp RT-qPCR analysis. A representative experiment (with technical triplicates) is shown.
